Fluorescence Microscopy Techniques
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RM21 Microscope
RM21 MicroMirror TIRF
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Fluorescence Microscopy Technique Provides New Views of Biological Process
Total internal reflection fluorescence (TIRF) microscopy is a well-known technique for single molecule microscopy. One of the challenges of dichroic-based TIRF is improving the signal-to-noise ratio with multiple wavelength experiments. We present in this paer an overview of MicroMirror TIRF where excitation and emission pathways are spatially, rather than spectrally, separated yielding improved signal-to-noise ratios.
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