Single Molecule Microscopy
>>TIRF Lock™
Related Products
RM21™ Microscope
Micro-Mirror TIRF System
TIRF Module
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Product Description |
After the user sets up TIR illumination and establishes the desired sample Z position, TIRF Lock™ keeps the sample in the focal plane by measuring the displacement of the exiting TIRF beam and maintaining that position via software feedback. The TIRF Lock™ system includes a quadrant photo-diode (QPD) head, a TIRF Lock™ controller, and a LabVIEW based VI. The TIRF Lock™ system is compatible with Mad City Labs RM21™ microscopes, the MicroMirror TIRF system, and the TIRF Module.
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As shown in the diagram above on the left, a small change in Z position of the sample due to differential thermal expansion in the microscope will result in a small change in position of the exit beam on the detector. The software uses that change in position to calculate an opposing change in the nanopositioner Z position, as shown in the schematic above on the right. |
Technical Specifications
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Detector Wavelength Range |
400nm-1000nm |
Detector Peak Responsivity
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0.4A/W @635nm |
0.67A/W @900nm |
Recommended Incident Beam Size |
300 µm to 1.5mmm |
Sensor Size |
2.4mm x 2.4mm |
Clear aperture diameter |
0.5 inches (1.25mm) |
Aperture thread |
SM05 (#0.535-40) |
Detector head mounting thread |
8-32 or M4 |
Mounting post holder thread |
1/4-20 or M6 (magnetic base and clamping fork provided) |
Detector head cable length |
6 feet (1.83m) |
Power supply |
90-260V |
Controller dimensions |
8.375 x 3.5 x 12 inches |
PC connections |
USB 2.0 |
Operating system compatibility |
(32 or 64 bit) Windows XP Pro/Vista/7/Window 8 |
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